Conformational constraints in nuclear DNA
نویسندگان
چکیده
منابع مشابه
Conformational constraints in nuclear DNA.
We have investigated DNA superstructure in a wide range of nuclei of higher cells by gently lysing cells to release structures that resemble nuclei but are depleted of nuclear proteins. The sedimentation properties of these structures, which we call nucleoids, have been examined in sucrose gradients containing the intercalating agent, ethidium. The sedimentation rate of nucleoids derived from t...
متن کاملConformational flexibility of DNA.
Pulsed Electron-Electron Double Resonance (PELDOR) on double-stranded DNA (ds-DNA) was used to investigate the conformational flexibility of helical DNA. Stretching, twisting, and bending flexibility of ds-DNA was determined by incorporation of two rigid nitroxide spin labels into a series of 20 base pair (bp) DNA duplexes. Orientation-selective PELDOR experiments performed at both X-band (9 GH...
متن کاملConformational changes induced by salt in complexes of histones and superhelical nuclear DNA.
When HeLa cells are lysed in solutions containing a non-ionic detergent and 0.75 M-NaCl, structures are released that retain many of the morphological features of nuclei. These nucleoids contain all the nuclear DNA, RNA and the 'core' histones, but few other proteins characteristic of chromatin. Their DNA is intact. The core histones dissociate on raising the salt concentration. We have probed ...
متن کاملAtrazine in sub-acute exposure results in sperm DNA disintegrity and nuclear immaturity in rats
This study was designed to evaluate the detrimental effect of atrazine (ATR) on germinal epitheliums (GE) cytoplasmic carbohydrate (CH) and unsaturated fatty acids (UFA) ratio and to clarify the effect of ATR on serum levels of FSH, LH, testosterone and inhibin-B (INH-B). The impact of ATR exposure on total antioxidant capacity (TAC), sperm DNA packing and integrity were also investigated. Seve...
متن کاملConformational fluctuations of DNA helix.
Treatment of a homogeneous sample of circular DNA molecules that contain at least one single-strand scission per molecule with ligase yields a population of covalently closed circles heterogeneous in the topological winding number alpha. Under proper conditions, the product molecules with different values of alpha can all be resolved by gel electrophoresis. The distributions in alpha for severa...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Science
سال: 1976
ISSN: 1477-9137,0021-9533
DOI: 10.1242/jcs.22.2.287